Tolbutamide induces conformational change and promotes albumin glycation

TitleTolbutamide induces conformational change and promotes albumin glycation
Publication TypeJournal Article
Year of Publication2015
AuthorsBansode, SB, Batkulwar, KB, Warkad, SD, Jana, AK, Sengupta, N, Kulkarni, MJ
JournalRSC Advances
Volume5
Issue50
Pagination40070-40075
Date PublishedAPR
ISSN2046-2069
Abstract

Tolbutamide is a first generation sulfonylurea drug used for the treatment of type II diabetes. In the present study, the effect of tolbutamide on albumin conformation was investigated using BSA as a model protein. The binding of tolbutamide induced a conformational change in albumin, which was confirmed by thioflavin T assay, ANS assay, and CD spectroscopy. Computer simulations suggested that the binding of tolbutamide increases the solvent accessibility of lysine residues, the hotspots of glycation. Furthermore, the change in conformation of albumin facilitates increased glycation, which was observed by AGE specific fluorescence and the results were corroborated by mass spectrometric analysis. This study suggested that tolbutamide enhances albumin glycation by inducing conformational change in BSA and hence could be a potential risk factor if used for a prolonged period.

DOI10.1039/c5ra03839a
Type of Journal (Indian or Foreign)

Foreign

Impact Factor (IF)

3.289

Divison category: 
Biochemical Sciences
Physical and Materials Chemistry