Synthesis and structural studies of S-type/N-type-locked/frozen nucleoside analogues and their incorporation in RNA-selective, nuclease resistant 2 `-5 ` linked oligonucleotides

TitleSynthesis and structural studies of S-type/N-type-locked/frozen nucleoside analogues and their incorporation in RNA-selective, nuclease resistant 2 `-5 ` linked oligonucleotides
Publication TypeJournal Article
Year of Publication2013
AuthorsErande, ND, Gunjal, AD, Fernandes, M, Gonnade, RG, Kumar, VA
JournalOrganic & Biomolecular Chemistry
Volume11
Issue5
Pagination746-757
Date PublishedNOV
ISSN1477-0520
Abstract

2'-endo locked or frozen (S-type)/3'-endo locked or frozen (N-type) nucleoside analogues were synthesized. Conformational analysis based on (3)J(HH) and NOE measurements is presented which is further confirmed by X-ray crystal structural studies. 2'-5' isoDNA oligonucleotides (ON) were synthesized using these modified nucleoside analogues and UV-T-m studies of the resultant 2'-5' isoDNA : RNA duplexes reflect the site-and sequence-dependent effects and confirm that the S-type sugar conformations were preferred over the N-type sugar geometry in such duplexes.

DOI10.1039/c2ob26762d
Type of Journal (Indian or Foreign)

Foreign

Impact Factor (IF)

3.487

Divison category: 
Center for Material Characterization (CMC)
Organic Chemistry