Potential dual role of eugenol in inhibiting advanced glycation end products in diabetes: proteomic and mechanistic insights

TitlePotential dual role of eugenol in inhibiting advanced glycation end products in diabetes: proteomic and mechanistic insights
Publication TypeJournal Article
Year of Publication2016
AuthorsSingh, P, Jayaramaiah, RH, Agawane, SB, Vannuruswamy, G, Korwar, AM, Anand, A, Dhaygude, VS, Shaikh, ML, Joshi, RS, Boppana, R, Kulkarni, MJ, Thulasiram, HV, Giri, AP
JournalScientific Reports
Volume6
Date PublishedJAN
ISSN2045-2322
Abstract

Medicinally important genus Ocimum harbors a vast pool of chemically diverse metabolites. Current study aims at identifying anti-diabetic candidate compounds from Ocimum species. Major metabolites in O. kilimandscharicum, O. tenuiflorum, O. gratissimum were purified, characterized and evaluated for anti-glycation activity. In vitro inhibition of advanced glycation end products (AGEs) by eugenol was found to be highest. Preliminary biophysical analysis and blind docking studies to understand eugenol-albumin interaction indicated eugenol to possess strong binding affinity for surface exposed lysines. However, binding of eugenol to bovine serum albumin (BSA) did not result in significant change in secondary structure of protein. In vivo diabetic mice model studies with eugenol showed reduction in blood glucose levels by 38% likely due to inhibition of alpha-glucosidase while insulin and glycated hemoglobin levels remain unchanged. Western blotting using anti-AGE antibody and mass spectrometry detected notably fewer AGE modified peptides upon eugenol treatment both in vivo and in vitro. Histopathological examination revealed comparatively lesser lesions in eugenol-treated mice. Thus, we propose eugenol has dual mode of action in combating diabetes; it lowers blood glucose by inhibiting a-glucosidase and prevents AGE formation by binding to epsilon-amine group on lysine, protecting it from glycation, offering potential use in diabetic management.

DOI10.1038/srep18798
Type of Journal (Indian or Foreign)

Foreign

Impact Factor (IF)

5.228

Divison category: 
Biochemical Sciences
Organic Chemistry