Optimization and scale up of production of alkaline protease from Conidiobolus coronatus

TitleOptimization and scale up of production of alkaline protease from Conidiobolus coronatus
Publication TypeJournal Article
Year of Publication2005
AuthorsLaxman, RSeeta, Sonawane, AP, More, SV, Rao, BS, Rele, MV, Jogdand, VV, Deshpande, VV, Rao, MB
JournalProcess Biochemistry
Volume40
Issue9
Pagination3152-3158
Date PublishedSEP
Type of ArticleArticle
ISSN1359-5113
KeywordsAlkaline Protease, Conidiobolus, Fermentation, Optimization, scale up
Abstract

Alkaline protease secreted by Conidiobolus coronatus has been evaluated extensively in tanneries and finds application in pre-tanning operations in leather manufacture. It is important to produce the enzyme in inexpensive and optimized media on large scale for the process to be commercially viable. The present paper describes optimization of fermentation conditions in shake flasks and scale up of production to 100 L in fermentors. The production is complete in 2-3 days comparable to bacterial fermentations. The organism utilized several carbon sources such as starch, sucrose, lactose, glucose and fructose for production. Soyabean meal at an optimum concentration of 2-3% was found to be best inducer. Diammonium hydrogen phosphate, casamino acids and Hi-media peptone gave activities comparable to yeast extract. Preservation and stabilization studies showed that glycerol conferred considerable stabilization at room temperature while ammonium sulphate precipitated enzyme at 0.9 saturation was best with stability up to 2 years even at room temperature. (c) 2005 Elsevier Ltd. All rights reserved.

DOI10.1016/j.procbio.2005.04.005
Type of Journal (Indian or Foreign)

Foreign

Impact Factor (IF)

2.529

Divison category: 
Biochemical Sciences