Lannea coromandelica attenuates glucagon and oxyntomodulin mediated cAMP formation in HEK cells stably-expressing human glucagon receptor

TitleLannea coromandelica attenuates glucagon and oxyntomodulin mediated cAMP formation in HEK cells stably-expressing human glucagon receptor
Publication TypeJournal Article
Year of Publication2015
AuthorsWalunj, S, Gupta, R, Joshi, SP, Sabharwal, SG, Joshi, K
JournalJournal of Herbal Medicine
Volume5
Issue3
Pagination153-157
Date PublishedSEP
ISSN2210-8033
KeywordscAMP, Diabetes, Glucagon, Lannea coromandelica, Mechanism, Oxyntomodulin
Abstract

Antagonism of glucagon is a potential therapeutic approach for Type 2 diabetes mellitus (T2DM). The bark extract of Lannea coromandelica has been reported to have hypoglycemic or anti-diabetic effects on animal models and human subjects. The aim of this study was to elucidate the molecular mechanisms of L. coromandelica extract as a glucagon receptor antagonist. The aqueous extract of L. coromandelica bark attenuated glucagon-mediated cAMP formation in a concentration dependent manner with an IC50 value of 4.24 +/- 1.31 mu g/ml. It shifted a concentration response curve of glucagon towards the right with a 4, 21 and 40-fold change in IC50 at 6.25, 12.5 and 25 mu g/ml of extract, respectively. Additionally, it attenuated an oxyntomodulin-mediated cAMP response. The extract was found specifically to inhibit glucagon mediated cAMP formation since it was ineffective in reducing cAMP levels nonspecifically-elevated by forskolin. The data support that the aqueous extract of L. coromandelica antagonizes the glucagon receptor and has the potential to reduce glucagon mediated liver glucose output. (C) 2015 Elsevier GmbH. All rights reserved.

DOI10.1016/j.hermed.2015.06.005
Type of Journal (Indian or Foreign)

Foreign

Impact Factor (IF)

1.2

Divison category: 
Chemical Engineering & Process Development