Comparative functional characterization of eugenol synthase from four different ocimum species: implications on eugenol accumulation

TitleComparative functional characterization of eugenol synthase from four different ocimum species: implications on eugenol accumulation
Publication TypeJournal Article
Year of Publication2016
AuthorsAnand, A, Jayaramaiah, RH, Beedkar, SD, Singh, PA, Joshi, RS, Mulani, FA, Dholakia, BB, Punekar, SA, Gade, WN, Thulasiram, HV, Giri, AP
JournalBiochimica Et Biophysica Acta-Proteins and Proteomics
Volume1864
Issue11
Pagination1539-1547
Date PublishedNOV
Abstract

Isoprenoids and phenylpropanoids are the major secondary metabolite constituents in Ocimum genus. Though enzymes from phenylpropanoid pathway have been characterized from few plants, limited information exists on how they modulate levels of secondary metabolites. Here, we performed phenylpropanoid profiling in different tissues from five Ocimum species, which revealed significant variations in secondary metabolites including eugenol, eugenol methyl ether, estragole and methyl cinnamate levels. Expression analysis of eugenol synthase (EGS) gene showed higher transcript levels especially in young leaves and inflorescence; and were positively correlated with eugenol contents. Additionally, transcript levels of coniferyl alcohol acyl transferase, a key enzyme diverting pool of substrate to phenylpropanoids, were in accordance with their abundance in respective species. In particular, eugenol methyl transferase expression positively correlated with higher levels of eugenol methyl ether in Ocimum tenuiflorum. Further, EGSs were functionally characterized from four Ocimum species varying in their eugenol contents. Kinetic and expression analyses indicated, higher enzyme turnover and transcripts levels, in species accumulating more eugenol. Moreover, biochemical and bioinformatics studies demonstrated that coniferyl acetate was the preferred substrate over coumaryl acetate when used, individually or together, in the enzyme assay. Overall, this study revealed the preliminary evidence for varied accumulation of eugenol and its abundance over chavicol in these Ocimum species. Current findings could potentially provide novel insights for metabolic modulations in medicinal and aromatic plants. (C) 2016 Elsevier B.V. All rights reserved.

DOI10.1016/j.bbapap.2016.08.004
Type of Journal (Indian or Foreign)

Foreign

Impact Factor (IF)

3.016

Divison category: 
Biochemical Sciences
Organic Chemistry