Chemical and biological metal nanoparticles as antimycobacterial agents: a comparative study

TitleChemical and biological metal nanoparticles as antimycobacterial agents: a comparative study
Publication TypeJournal Article
Year of Publication2015
AuthorsSingh, R, Nawale, LU, Arkile, MA, Shedbalkar, UU, Wadhwani, SA, Sarkar, D, Chopade, BAnanda
JournalInternational Journal of Antimicrobial Agents
Date PublishedAUG
KeywordsAntibiotic resistance, Antimycobacterial agent, Cytotoxicity, Mycobacteria, Nanoparticles, tuberculosis

Resistance among mycobacteria leading to multidrug-resistant and extensively drug-resistant tuberculosis is a major threat. However, nanotechnology has provided new insights in drug delivery and medicine development. This is the first comparative report to determine the activity of chemically and biologically synthesised silver nanoparticles (AgNPs) and gold nanoparticles (AuNPs) against mycobacteria. Screening data revealed the high mycobactericidal efficiency of AgNPs, with minimum inhibitory concentrations (MICs) of <3 mu g/mL, whereas no such activity was exhibited by AuNPs at concentrations up to 100 mu g/mL. Moreover, in vitro and ex vivo THP-1 infection model assays showed greater efficacy of chemical AgNPs compared with biogenic AgNPs to inhibit active and dormant stage mycobacterial growth. Up to 40% cytotoxicity against human cell lines was observed at a AgNP concentration of 10 x MIC (30 mu g/mL) after 48 h. AgNPs were shown to have more specificity towards mycobacteria than towards other Gram-negative and Gram-positive pathogenic bacteria. The selectivity index was found to be in the range of 11-23, indicating the potential of these nanoparticles for use in developing new therapeutics for tuberculosis. (C) 2015 Elsevier B.V. and the International Society of Chemotherapy. All rights reserved.

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Organic Chemistry