Carbon nanotube-modified sodium dodecyl sulfate-polyacrylamide gel electrophoresis for molecular weight determination of proteins

TitleCarbon nanotube-modified sodium dodecyl sulfate-polyacrylamide gel electrophoresis for molecular weight determination of proteins
Publication TypeJournal Article
Year of Publication2011
AuthorsParthasarathy, M, Debgupta, J, Kakade, BA, Ansary, AAyoobul, Khan, MIslam, Pillai, VK
JournalAnalytical Biochemistry
Volume409
Issue2
Pagination230-235
Date PublishedFEB
ISSN0003-2697
KeywordsCarbon nanotubes, Gel electrophoresis, Molecular weight determination, Polyacrylamide, Protein separation
Abstract

The effect of incorporating carbon nanotubes (CNTs) in the gel matrix on the electrophoretic mobility of proteins based on their molecular weight differences was investigated using sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE). More specifically, a reduction in standard deviation in the molecular weight calibration plots by 55% in the case of multiwalled carbon nanotubes (MWCNTs) and by 34% in the case of single-walled carbon nanotubes (SWCNTs) compared with that of pristine polyacrylamide gels was achieved after incorporating an insignificant amount of functionalized CNTs into the gel matrix. A mechanism based on a more uniform pore size distribution in CNT modified polyacrylamide gel matrix is proposed. Furthermore, the impact of SWCNTs and MWCNTs on the mobility of proteins in different molecular weight regimes at a given acrylamide concentration offers a tunable gel matrix in terms of the selection of molecular weight ranges of proteins. The robustness and excellent reproducibility of the CNT-PAGE protocol are expected to have a significant impact on the molecular weight determination of newly isolated proteins. (C) 2010 Elsevier Inc. All rights reserved.

DOI10.1016/j.ab.2010.10.021
Type of Journal (Indian or Foreign)

Foreign

Impact Factor (IF)

2.85

Divison category: 
Biochemical Sciences
Physical and Materials Chemistry