Brevibacillus sp: a novel thermophilic source for the production of bile salt hydrolase

TitleBrevibacillus sp: a novel thermophilic source for the production of bile salt hydrolase
Publication TypeJournal Article
Year of Publication2009
AuthorsSridevi, N, Prabhune, A
JournalApplied Biochemistry and Biotechnology
Volume157
Issue2
Pagination254-262
Date PublishedMAY
ISSN0273-2289
KeywordsBile salt hydrolase, Brevibacillus sp, Fermentation, Glycodeoxycholic acid, Hot springs, Thermophile
Abstract

A thermophilic microorganism growing within the temperature range of 40-65 A degrees C (optimum at 55 A degrees C) was isolated from hot water springs near Konkan, Maharashtra, India. Based on 16S rDNA sequence analysis, it was concluded that the isolate belongs to the genus Brevibacillus. The present paper reports the isolation, identification, and standardization of fermentation conditions for the production of enzyme, bile salt hydrolase (EC 3.5.1.24) which is produced intracellularly at high temperatures. This is the first report regarding the production of bile salt hydrolase from a thermophilic source. Optimization of fermentation conditions resulted in a 2.9-fold enhancement in enzyme production.

DOI10.1007/s12010-008-8326-9
Type of Journal (Indian or Foreign)

Foreign

Impact Factor (IF)

1.879

Divison category: 
Biochemical Sciences