1H, 13C and 15N NMR assignments of two plant protease inhibitors (IRD7 and IRD12) from the plant capsicum annuum.

Title1H, 13C and 15N NMR assignments of two plant protease inhibitors (IRD7 and IRD12) from the plant capsicum annuum.
Publication TypeJournal Article
Year of Publication2018
AuthorsGartia, J, Barnwal, RP, Anangi, R, Giri, AR, King, G, Chary, KVR
JournalBiomolecular NMR Assignments
Volume13
Issue1
Pagination31-35
Date PublishedSEP
Type of ArticleArticle
ISSN1874-2718
KeywordsBt transgenic, Hetero-nuclear NMR, inhibitory repeating domains, Protease inhibitors (PIs), Resonance assignments
Abstract

Helicoverpa species are polyphagous pests, with the larval stages causing major damage to economically valuable crops such as cotton, tomato, corn, sorghum, peas, sunflower, wheat and other pulses. Over the years, Helicoverpa armigera has developed resistance to most classes of chemical insecticides, and consequently it is now largely controlled on cotton plants via the use of Bt transgenic crops that express insecticidal Cry toxins which in-turn expedited resistance development in a number of pest species including H. armigera. In a hope to provide other eco-friendly alternatives solutions to counter the effect of the pest, people have identified a number of protease inhibitors (PIs) from the domesticated capsicum species Capsicum annuum, several of which potently inhibited H. armigera gut proteases and impeded growth of H. armigera larva. With a view to explore and enhance the specific nature or properties of these PIs on the mechanism of inhibition, structural and functional characterization of these PIs are inevitable. Towards this goal, we have carried out complete 1H, 13C and 15N resonance assignments of two of these PIs, identified as IRD7 and IRD12, using a suite of 2D and 3D multi-dimensional and multi-nuclear NMR experiments.

DOI10.1007/s12104-018-9846-z
Type of Journal (Indian or Foreign)

Foreign

Impact Factor (IF)

0.593

Divison category: 
Biochemical Sciences